溃疡性结肠炎
先天性淋巴细胞
克罗恩病
炎症性肠病
医学
疾病
内科学
免疫学
胃肠病学
淋巴系统
流式细胞术
炎症
结肠炎
先天免疫系统
受体
作者
Marianne Forkel,Sophie van Tol,Charlotte Höög,Jakob Michaëlsson,Sven Almér,Jenny Mjösberg
出处
期刊:Journal of Crohn's and Colitis
[Oxford University Press]
日期:2018-11-29
卷期号:13 (1): 67-78
被引量:86
标识
DOI:10.1093/ecco-jcc/jjy119
摘要
Innate lymphoid cells [ILC] have been suggested to play a role in inflammatory bowel disease [IBD]. Here, we investigated the ILC compartment in intestinal biopsies and blood from distinct patient groups with Crohn's disease [CD] and ulcerative colitis [UC], either newly diagnosed or with disease established for at least 1 year. This approach allowed us to simultaneously investigate temporal, disease-specific, and tissue-specific changes in ILC composition in IBD.ILC subset frequencies, phenotype, and transcription factor profile in blood and intestinal biopsies were investigated by multi-parameter flow cytometry analysis. Endoscopic disease severity was judged using the ulcerative colitis endoscopic index of severity and the simple endoscopic score for Crohn's disease.The frequency of NKp44+ILC3 was decreased in inflamed tissue, both in patients with CD and those with UC, already at the time of diagnosis, and correlated with disease severity. Simultaneously, the frequency of ILC1 was increased in patients with CD, whereas the frequency of ILC2 was increased in patients with UC. However, in patients with established UC or CD, both ILC1 and ILC2 were increased. In contrast to the ILC composition in inflamed tissue, ILC in non-inflamed tissue or blood were unchanged compared with non-IBD controls. Finally, in patients undergoing treatment with an anti-α4β7 antibody the frequencies of ILC in peripheral blood remained unchanged.We report both shared and distinct changes in ILC composition depending on diagnosis and disease duration. The alterations in ILC composition in IBD occur selectively at inflamed sites in the gut.
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