生物
粉虱
保幼激素
基因敲除
RNA干扰
生殖力
铁蛋白
基因
若虫
昆虫
转录因子
半翅目
细胞生物学
遗传学
植物
核糖核酸
生物化学
人口
人口学
社会学
作者
Huilin Yu,Kui Wang,Zhifang Yang,Xiang Li,Shunxiao Liu,Liuhao Wang,Hongwei Zhang
出处
期刊:Environmental Entomology
[Oxford University Press]
日期:2023-06-15
卷期号:52 (4): 750-758
摘要
Ferritins are conserved iron-binding proteins that exist in most living organisms and play an essential role in the maintenance of cellular iron homeostasis. Although ferritin has been studied in many species, little is known about its role in the whitefly, Bemisia tabaci. In this study, we identified an iron-binding protein from B. tabaci and named it BtabFer1. The full-length cDNA of BtabFer1 is 1,043 bp and encodes a protein consisting of 224 amino acids with a deduced molecular weight of 25.26 kDa, and phylogenetic analysis shows that BtabFer1 is conserved among Hemiptera insects. The expression levels of BtabFer1 in different developmental stages and tissues were analyzed by real-time PCR, and results showed that BtabFer1 was ubiquitously expressed at all developmental stages and in all examined tissues. The RNAi-mediated knockdown of BtabFer1 caused a significant reduction in survival rate, egg production, and egg hatching rate of whiteflies. Knockdown of BtabFer1 also inhibited the transcription of genes in the juvenile hormone signal transduction pathway. Taken together, these results suggest that BtabFer1 plays a critical role in the development and reproduction of whiteflies. This study can broaden our understanding of ferritin in insect fecundity and development, as well as provide baseline data for future studies.
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