微流控
细胞包封
纳米技术
永生化细胞系
再生医学
微球
分散性
封装(网络)
胶囊
胚胎干细胞
细胞培养
微流控芯片
材料科学
移植
生物医学工程
组织工程
化学
细胞生物学
干细胞
计算机科学
生物
医学
计算机网络
工程类
遗传学
生物化学
植物
外科
化学工程
高分子化学
基因
作者
Victoria L. Workman,Stephen B. Dunnett,Peter Kille,Daniel D. Palmer
出处
期刊:Biomicrofluidics
[American Institute of Physics]
日期:2007-03-01
卷期号:1 (1): 014105-014105
被引量:62
摘要
Cellular transplantation is a promising technology with great clinical potential in regenerative medicine and disease management. However, effective control over patient immunological response is essential. The encapsulation of cells within hydrogel microspheres is an increasingly prevalent method for the protection of cellular grafts from immune rejection. Microfluidic “chip” reactors present elegant solutions to several capsule generation issues, including the requirement for intercapsule uniformity, high reproducibility, and sterile, good manufacturing practice compliance. This study presents a novel method for the on-chip production of stable, highly monodisperse alginate microspheres and demonstrates its utility in the encapsulation of an immortalized human-derived cell line. Four populations of immortalized human embryonic kidney cells (HEK293) were encapsulated on chip within monodisperse alginate capsules. Cell viability measurements were recorded for each of the four encapsulated populations for 90days.
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