合子性
微卫星
聚合酶链反应
遗传学
生物
血清学
单卵双胞胎
计算生物学
抗体
基因
等位基因
作者
Albert J. Becker,Andreas Busjahn,Hans-Dieter Faulhaber,Sylvia Bähring,James Robertson,Herbert Schuster,F. C. Luft
出处
期刊:PubMed
日期:1997-05-01
卷期号:42 (5): 260-6
被引量:30
摘要
Twin zygosity determinations can be performed with anthropologic, serologic and genetic markers; however, these methods are more than occasionally inefficient, often expensive and sometimes inaccurate. We used microsatellites as DNA markers and developed a largely automated, rapid and efficient method of determining zygosity.We used five highly polymorphic short tandem repeat loci, coamplified by polymerase chain reaction (PCR) using fluorescence-labeled primers. Thirty-six samples were simultaneously analyzed by electrophoresis and laser detection. The PCR products were sized by automated fragment analysis.We typed 132 pairs of monozygotic (MZ) and dizygotic (DZ) twins. With five markers, the probability that any twin pair was MZ if all markers were concordant was 99%.This method is a rapid and reliable approach to zygosity detection.
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