念珠菌
生物
立克次体
蓖麻硬蜱
基因
遗传学
16S核糖体RNA
病毒学
滴答声
病毒
作者
Yana Igolkina,V. A. Rar,Valeriy V. Yakimenko,Artem Tikunov,Nina V. Tikunova
标识
DOI:10.1016/j.ttbdis.2022.101933
摘要
The spotted fever group is the largest group in the Rickettsia genus, and new candidate species of this group are found throughout the world. "Candidatus Rickettsia thierseensis" was observed in a single Ixodes ricinus in Austria in 2020. However, sequences of five genes (total length: 5305 bp) of the "Candidatus R. thierseensis" were nearly identical to those available for "Candidatus Rickettsia uralica" identified in Ixodes trianguliceps and small mammals in Russia in 2015. To confirm the identity of these species, appropriate fragments of seven genetic loci from four isolates of "Candidatus R. uralica" were sequenced and compared with the "Candidatus R. thierseensis" sequences. Additionally, the groEL gene fragment was first sequenced for "Candidatus R. uralica". In this analysis, "Candidatus R. uralica" and "Candidatus R. thierseensis" demonstrated 100% identity in the 23S-5S IGS region (354 bp) and in fragments of the 16S rRNA (991 bp), gltA (1119 bp), and htrA (463 bp) genes, differing by only one substitution in each of the ompA (3696 bp), ompB (4871 bp), and sca4 (2971 bp) gene fragments. Thus, "Candidatus R. uralica" and "Candidatus R. thierseensis" are likely the same species based on comparative sequence analysis.
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