生长素
根毛
生物
突变体
野生型
水稻
细胞生物学
侧根
延伸率
活性氧
生物化学
植物
拟南芥
基因
冶金
材料科学
极限抗拉强度
作者
Chenliang Yu,Chendong Sun,Chenjia Shen,Suikang Wang,Fang Liu,Yan Liu,YunLong Chen,Chuanyou Li,Qian Qian,Bibek Aryal,Markus Geisler,De An Jiang,Yanhua Qi
出处
期刊:Plant Journal
[Wiley]
日期:2015-07-22
卷期号:83 (5): 818-830
被引量:133
摘要
Auxin and cadmium (Cd) stress play critical roles during root development. There are only a few reports on the mechanisms by which Cd stress influences auxin homeostasis and affects primary root (PR) and lateral root (LR) development, and almost nothing is known about how auxin and Cd interfere with root hair (RH) development. Here, we characterize rice osaux1 mutants that have a longer PR and shorter RHs in hydroponic culture, and that are more sensitive to Cd stress compared to wild-type (Dongjin). OsAUX1 expression in root hair cells is different from that of its paralogous gene, AtAUX1, which is expressed in non-hair cells. However, OsAUX1, like AtAUX1, localizes at the plasma membrane and appears to function as an auxin tranporter. Decreased auxin distribution and contents in the osaux1 mutant result in reduction of OsCyCB1;1 expression and shortened PRs, LRs and RHs under Cd stress, but may be rescued by treatment with the membrane-permeable auxin 1-naphthalene acetic acid. Treatment with the auxin transport inhibitors 1-naphthoxyacetic acid and N-1-naphthylphthalamic acid increased the Cd sensitivity of WT rice. Cd contents in the osaux1 mutant were not altered, but reactive oxygen species-mediated damage was enhanced, further increasing the sensitivity of the osaux1 mutant to Cd stress. Taken together, our results indicate that OsAUX1 plays an important role in root development and in responses to Cd stress.
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