Quantitative determination of puerarin in Pueraria lobata by HPLC and elementary study on their fluorescence

OBJECTIVETo set up an optimal HPLC method to determine the content of puerarin from Pueraria lobata. METHODSThe contents of this compound in the Pueraria lobata were determined by reversed-phase liquid chromatography.BDS HYPERSIL C_(18 )column(250 mm×(4.6) mm,5 μm)was used,ethanol-water containing (0.2%) phosphatic acid( 15∶85) as the mobile phase and the fluorescence of the puerarin solution was concerned, flow rate was (1.0) mL·min~(-1),and fluorescence detector: ex:260 nm,em:472 nm. RESULTSThe good linear relationships were obtained under the optimum conditions for each component. The recoveries of the standards added were (97.1%)-(101.7%) while the relatively standard deviations were within the range of (0.62%) to (1.61%). CONCLUSIONThis method is accurate and reliable for the quantitive analysis of puerarin in Pueraria lobata.