基因传递
纳米金刚石
转染
纳米技术
遗传增强
聚合物
细胞毒性
表面改性
材料科学
化学
基因
体外
生物化学
有机化学
物理化学
钻石
作者
Xuan Zhang,Mark I-Cheng Chen,Robert Lam,Xiaoyang Xu,Eiji Osawa,Dean Ho
出处
期刊:ACS Nano
[American Chemical Society]
日期:2009-08-31
卷期号:3 (9): 2609-2616
被引量:339
摘要
Gene therapy holds great promise for treating diseases ranging from inherited disorders to acquired conditions and cancers. Nonetheless, because a method of gene delivery that is both effective and safe has remained elusive, these successes were limited. Functional nanodiamonds (NDs) are rapidly emerging as promising carriers for next-generation therapeutics with demonstrated potential. Here we introduce NDs as vectors for in vitro gene delivery via surface-immobilization with 800 Da polyethyleneimine (PEI800) and covalent conjugation with amine groups. We designed PEI800-modified NDs exhibiting the high transfection efficiency of high molecular weight PEI (PEI25K), but without the high cytotoxicity inherent to PEI25K. Additionally, we demonstrated that the enhanced delivery properties were exclusively mediated by the hybrid ND-PEI800 material and not exhibited by any of the materials alone. This platform approach represents an efficient avenue toward gene delivery via DNA-functionalized NDs, and serves as a rapid, scalable, and broadly applicable gene therapy strategy.
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