Therapeutic potential of Brazilian green propolis and oregano ( Origanum vulgare ) extracts in collagen hydrogel for pressure ulcer repair: an experimental study in an animal model

This study investigated the effects of incorporating Brazilian green propolis ethanolic extract and oregano (Origanum vulgare) methanolic extract into type I collagen hydrogel to enhance pressure ulcer healing in mice. Forty-four mice were divided into four groups: untreated control, plain collagen hydrogel, collagen hydrogel with 1% green propolis extract, and collagen hydrogel with 1% oregano extract. Pressure ulcers were induced on their dorsal skin, followed by a 14-day assessment of wound area, contraction, re-epithelialization, dermal collagen density, and levels of reactive oxygen species and nitrite. Results showed significant tissue repair promotion by both propolis and oregano extracts, with oregano exhibiting superior wound contraction. These hydrogels also facilitated skin layer reorganisation and reduced reactive oxygen species and nitrite production. Collagen hydrogels containing these extracts show promising therapeutic potential for pressure ulcer treatment.