Application of decellularized methods and decellularized matrix scaffolds in tissue engineering and regenerative medicine bring hope to solve the problems of organ and tissue reparation and reconstruction.The methods of decellularization can be summarized as chemical methods,physical methods and enzymatic methods.And it has successfully been used in a variety of tissue engi-neering and regenerative medicine.this article summarized the regular decellularized methods and its application in a variety of tissue engineering and regenerative medicine and analyzed on the basis of recent related literatures inside and outside.