SH-SY5Y型
神经毒性
神经毒素
帕金森病
化学
分子生物学
细胞生物学
生物
药理学
毒性
细胞培养
生物化学
医学
内科学
神经母细胞瘤
疾病
遗传学
有机化学
作者
Morteza Ahmadzadeh-Darinsoo,Saeed Abbasi,Ehsan Arefian,Carole Bernard,Azita Parvaneh Tafreshi
标识
DOI:10.1016/j.ncrna.2021.12.004
摘要
In the current study, the effects of 7-BIO as a specific GSK3β inhibitor was examined on cell survival and expression of miR-29a-3p and miR-34a-5p in neurotoxin MPP+ treated SH-SY5Y cells. Our findings revealed that while co-treatment of the cells with 7-BIO and MPP+ did not alter the toxicity induced by MPP+, pretreatment with 3.5 μM 7-BIO for 6 h increased the survival of the 2 mM MPP+ treated cells. Also, qRT-PCR analysis of gene expression showed that while miR-29a-3p was unchanged in cells treated with either 2 mM MPP+ or 3.5 μM 7-BIO alone, miR-34a-5p was increased by MPP+ but decreased by 7-BIO. Pretreatment with 3.5 μM 7-BIO prior to MPP+ however, increased miR-29a-3p but decreased miR-34a-5p induced by MPP+. We therefore suggest that 7-BIO inhibition of GSK3β alleviates the MPP+ induced neurotoxicity by regulating miR-29a-3p and miR-34a-5p expressions in Parkinson's disease model SH-SY5Y cells.
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