日本脑炎
表面增强拉曼光谱
病毒学
检出限
银纳米粒子
基质(水族馆)
病毒
病毒性脑炎
黄病毒
表面等离子共振
材料科学
拉曼光谱
脑炎
纳米技术
纳米颗粒
化学
医学
生物
色谱法
拉曼散射
光学
生态学
物理
作者
Manish Nath Tripathi,Kehar Singh,Umakant Yadav,Rohit Ranjan Srivastava,Mayank Gangwar,Gopal Nath,Preeti S. Saxena,Anchal Srivastava
标识
DOI:10.1016/j.antiviral.2022.105382
摘要
Japanese encephalitis (JE) is a mosquito-borne flavivirus infection named Japanese Encephalitis Virus (JEV), prevalent in Asia-pacific countries, requires an accurate and rapid diagnosis to contain the outbreak of the disease. In cases of low viral load in early-stage infections, this task becomes difficult. Therefore, we have developed a surface-enhanced Raman spectroscopy (SERS) based biosensor for rapid, sensitive, and early-stage detection of JE antigen. In this work, silver nanoparticles were deposited over a glass coverslip and used as a substrate for designing the sensing platform. Silver Nanoparticles have good metallic properties and plasmon activity. Therefore, it amplifies the Raman signals and provides a suitable surface for the SERS substrate. The developed platform has been used for the detection of the Japanese encephalitis virus (JEV). The fabricated sensor shows a linear response from 5 ng/mL to 80 ng/mL with a limit of detection (LoD) of ∼7.6 ng/mL. Therefore, this method could be a significant addition to the diagnostic modalities for early, sensitive, and specific diagnoses of JE antigen even at the nanogram level.
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