自愈水凝胶
软骨发生
间充质干细胞
软骨
II型胶原
软骨细胞
碱性磷酸酶
糖胺聚糖
化学
Ⅰ型胶原
组织工程
生物医学工程
细胞生物学
材料科学
解剖
病理
医学
生物
生物化学
高分子化学
酶
作者
Claire E. Kilmer,Carly M. Battistoni,Abigail Cox,Gert J. Breur,Alyssa Panitch,Julie C. Liu
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2020-05-11
卷期号:6 (6): 3464-3476
被引量:51
标识
DOI:10.1021/acsbiomaterials.9b01939
摘要
Collagen type II is a promising material to repair cartilage defects since it is a major component of articular cartilage and plays a key role in chondrocyte function. This study investigated the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (MSCs) embedded within a 3:1 collagen type I to II blend (Col I/II) hydrogel or an all collagen type I (Col I) hydrogel. Glycosaminoglycan (GAG) production in Col I/II hydrogels was statistically higher than that in Col I hydrogels or pellet culture, and these results suggested that adding collagen type II promoted GAG production. Col I/II hydrogels had statistically lower alkaline phosphatase (AP) activity than pellets cultured in a chondrogenic medium. The ability of MSCs encapsulated in Col I/II hydrogels to repair cartilage defects was investigated by creating two cartilage defects in the femurs of rabbits. After 13 weeks, histochemical staining suggested that Col I/II blend hydrogels provided favorable conditions for cartilage repair. Histological scoring revealed a statistically higher cartilage repair score for the Col I/II hydrogels compared to either the Col I hydrogels or empty defect controls. Results from this study suggest that there is clinical value in the cartilage repair capabilities of our Col I/II hydrogel with encapsulated MSCs.
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