Study on correlation between morphological change and intestinal permeability after gut barrier injury

Objective To observe the correlation between morphological change and intestinal permeability from gut barrier injury of the rats under stress.Method We made experimental rat model of hemorrhagic shock. The morphological change of intestinal mucosa were observed through light and electronic microscope, including histologic examination, determination of villous thickness and evaluation of mucosal damage index in 1,3,6,12 and 24 h after reinstitution, and lactulose mannitol ratio of urine was detected to analyze the change of intestinal mucosal barrier function for studying their relationship. Result The lesions in small intestine were even more obvious in intestinal mucosa, especially in 1 h; it began to reinstitution within 3 h, and most part of managed mucosa was near to reinstitution entirely and finished in 24 h, but villous thickness reduced progressively; Urine lactulose manrutol ratio in the experimental groups raised significantly, reached the peak in 3 and 6 h group respectively,and still increased slightly,compared with control group. Conclusion Intestinal mucosal barrier were subjected to serious damage under stress, the recovery of intestinal barrier function was obviously slower than that of intestinal mucosal morphological changes.There was no direct relationship between the intestinal permeability and its morphological changes.