[Determination of psoralen and isopsoralen in Psoralea corylifolia L. from different habitats].

OBJECTIVE: To study the psoralen and isopsoralen content in Psoralea corylifolia from different habitats. METHOD: C18 column was used. Mobile phase was methanol-water (45:55). The detection wavelength was at 245nm and the flow rate was 1.0 ml/min. RESULT: The linearity was obtained over the range of 0.448 to approximately 0.448 microg (r = 0.9999) for psoralen, 0.356 to approximately 0.356 microg = 0.997) for isopsoralen. CONCLUSION: The contents of psoralen and isopsoralen in Psoralea corylifolia from different habitats have no obvious difference.