医学
体内
活体显微镜检查
背
血管生成
病理
生物医学工程
血管内皮生长因子
再生(生物学)
皮肤修复
新生血管
离体
血管内皮生长因子受体
伤口愈合
川地31
微循环
解剖
生物
细胞生物学
外科
免疫组织化学
癌症研究
生物技术
放射科
作者
Yu‐Hang Liu,Lorenz M. Brunner,Johannes Rebling,Maya Ben‐Yehuda Greenwald,Sabine Werner,Michael Detmar,Daniel Razansky
出处
期刊:Theranostics
[Ivyspring International Publisher]
日期:2022-01-01
卷期号:12 (2): 558-573
被引量:15
摘要
Background: Microcirculation is essential for skin homeostasis and repair. A variety of growth factors have been identified as important regulators of wound healing. However, direct observation and longitudinal monitoring of skin remodeling in an unperturbed in vivo environment remains challenging. Methods: We report on non-invasive longitudinal imaging of the wound healing process in transgenic mice overexpressing vascular endothelial growth factor A (VEGF-A) in keratinocytes by means of large-scale optoacoustic microscopy (LSOM). This rapid, label-free, high throughput intravital microscopy method averts the use of dorsal skin-fold chambers, allowing for fully non-invasive repeated imaging of intact wounds with capillary resolution over field-of-view spanning several centimeters. Results: We observed VEGF-driven enhancement of dermal vascularization in ears, dorsal skin and healing wounds and quantified the hemoglobin content, fill fraction, vessel diameter and tortuosity. The in vivo findings were further corroborated by detailed side-by-side classical histological whole-mount vascular stainings and pan-endothelial CD31 immunofluorescence. Conclusion: The new approach is suitable for supplementing or replacing the cumbersome histological procedures in a broad range of skin regeneration and tissue engineering applications.
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