Study on“kidney corresponding with winter” by differential expression gene in testicle in seasons

Objective To screen gene fragment differentially expressed in testicle in winter and summer. Method Differential display reverse transcription PCR (DDRT-PCR) was used to detect the total RNA in testicle sample. Results ①Forty-four gene fragments differentially expressed were obtained. Ten gene fragments sequence were detected and 7 of them had higher homology with known genes. ②Three new genes were discovered and were included by gene bank (CK743226,CK743227,CK743228). Conclusion ①In physiological situation, seasonal variation of winter and summer can result in change of gene expression in testical. ②Conarium regulats gonad according to season by differential expression of gene. ③Three new genes may be needed by animals for self-regulation according to season and for evolvement . ④The theory of “kidney corresponding with winter” has substance basis. Its regulating mechanism is related to conarium’s regulation to differential expression of gene in testical.