13C CHEMICAL SHIFTS OF SOLID (1→4)-α- d -GLUCANS BY CROSS POLARIZATION/MAGIC ANGLE SPINNING (CP/MAS) NMR SPECTROSCOPY. CONFORMATION-DEPENDENT 13C CHEMICAL SHIFT AS A REFERENCE IN DETERMINING CONFORMATION IN AQUEOUS SOLUTION

Abstract 13C chemical shifts of linear and cyclic amyloses in solid state were measured by cross polarization/magic angle spinning (CP/MAS) NMR spectroscopy. It is found that rapid conformational isomerism occurs in aqueous solution for amylose and its linear oligomer with lifetime <10−3s with reference to the conformation-dependent 13C chemical shifts obtained in solid state.