Three-dimensional ordered macroporous magnetic photonic crystal microspheres for enrichment and detection of mycotoxins (I): Droplet-based microfluidic self-assembly synthesis

Ordered porous materials are attracting enormous attention due to their uniform pore structures, particularly the magnetic photonic crystal microspheres (PCMs) which not only possess unique photonic crystal structure but also can achieve separation easily based on magnet. Here, a two-phase microfluidic self-assembly synthetic system was established simply and employed for the preparation of three dimensional PCMs (3DPCMs) by using the emulsion droplet approach. One phase (dispersed phase) was an aqueous emulsion containing Fe3O4, silica (SiO2) and polystyrene (PS) nanoparticles; another phase (continuous phase) was pure silicone oil. The droplets were formed by introducing the dispersed phase into the continuous phase through a tee valve. By heating the droplets, the water would evaporate and the nanoparticles would finally assemble into solid microspheres, which could be changed into macroporous 3DPCMs after removal of the PS nanoparticles by calcination. The contents and particle sizes of Fe3O4, SiO2 and PS nanoparticles in the dispersed phase were investigated in detail and optimized to prepare macroporous magnetic 3DPCMs with high quality. The morphologies, surface crystal structure, magnetic property, particle size distribution, specific surface area and pore size of the macroporous magnetic 3DPCMs were characterized. The expected 3DPCM displayed regular and uniform photonic crystal structure, narrow particle size distribution and strong magnetic property. The macroporous magnetic 3DPCMs grafted with vomitoxin (DON)-antibodies could be applied for selective enrichment of DON in real samples.