Objective Identifying the dominant Lactobacillus producing bacteriocin,which were screened from 60 healthy women vaginas,provides choice and credible strains for the research of probiotic.Methods 19 strains of Lactobacillus which produce bacteriocin and abundant lactic acid were screened by the method of Oxford-cup tests,and they were identified by several important biochemical characteristics.Results According to the results of these experiments,19 strains were identified that 9 were Lactobacillus.gasseri,1 was Lactobacillus.salivarius and 9 were Lactobacillus.crispatus.Conclusions These 19 strains Lactobacillus which were screened through this experiment were dominant beneficial bacteria in the healthy female vagina,which had high ability of producing acid and bacteriocin.16 strains of them produce H_2O_2 and some had great probiotic production value.