In order to study the effects of the expression of EPO on rat kidney during intermittent hypoxia training, this paper determines the expression of EPO mRNA by RT-PCR. The Results show that the expression of EPO is increased in a-cute hypoxia condition in 3000 m and 4000 m, however it can be upregulated during intermittent hypoxia training in 3000 m. The expression of EPO gene is increased much more during appropriate intermittent hypoxia and training.