嗜麦芽窄食单胞菌
脂肪酶
Plackett–伯曼设计
食品科学
酵母抽提物
琼脂平板
寡养单胞菌
琼脂
化学
生物强化
微生物学
细菌
生物
色谱法
微生物
假单胞菌
发酵
生物化学
酶
铜绿假单胞菌
遗传学
作者
Morvarid Hasan-Beikdashti,Hamid Forootanfar,Mohammad S. Safiarian,Atefeh Ameri,Mohammad Hossein Ghahremani,Mohammad Reza Khoshayand,Mohammad Ali Faramarzi
标识
DOI:10.1016/j.jtice.2012.03.005
摘要
The production of an extracellular lipase from the newly isolated Stenotrophomonas maltophilia was optimized via the statistical design method. During a screening program, the microorganism was isolated from soil samples, according to lipolytic activity on nutrient agar medium supplemented with Rhodamine B. It was identified using morphological and biochemical properties as well as 16S rDNA sequencing analysis. After the initial screening of 10 factors of the medium components, using Plackett–Burman design, results showed that four of them, included olive oil, peptone, yeast extract, and ferrous sulfate, exhibited significant effects on the lipase production by S. maltophilia. The level of each factor was subjected to optimization by using the Box–Behnken technique, and a 9.1-fold enhancement of lipase productivity (from 500 U/ml to 4559 U/ml) was achieved overall in the presence of optimum levels of the effective factors. Forty-three percent of capsaicin (initial concentration of 500 mg/L) was converted to vanillylamine after treatment with the optimized medium (lipase activity 4559 U/ml) for 18 h.
科研通智能强力驱动
Strongly Powered by AbleSci AI