Quantitative analysis of notoginsenoside R_1 in Radix Notoginseng by HPLC gradient elution mode

OBJECTIVE:To quantitate analyze notoginsenoside R 1 in Radix Notoginseng by HPLC gradient elution mode.METHODS:The chromatographic conditions included column Sphericorb NH 2,mobile phase of acetonitrile methanol water(gradient concentration 55∶30∶15→70∶20∶10),and detector UV 210 nm.RESULTS:The linear range and the limit of detection for notoginsenoside R 1 were within 5～40 μg·ml -1 ( r = 0.999 4 )and above 0.6 μg·ml -1 of by measuring the peak area,respectively.The method was accurate (mean recovery= 102.6 %, RSD = 1.43 %).CONCLUSIONS:The method was proved to be simple and rapid. [