DNA Topoisomerase Protocols

Assaying DNA Topoisomerase I Relaxation Activity Lance Stewart and James J. Champoux DNA Topoisomerase II-Catalyzed DNA Decatenation Andrea Haldane and Daniel M. Sullivan Plasmid DNA Supercoiling by DNA Gyrase Penny J. Sayer, Martin L. Goble, Mark Oram, and L. Mark Fisher Analyzing Reverse Gyrase Activity Michel Duguet, Christine Jaxel, Anne-Ceile Declais, Fabrice Confalonieri, Janine Marsault, Claire Bouthier de la Tour, Marc Nadal, and Christiane Portemer Topoisomerase II-Catalyzed ATP Hydrolosis as Monitored by Thin-Layer Chromatography Paul S. Kingma, John M. Fortune, and Neil Osheroff Use of a Real-Time, Coupled Assay to Measure the ATPase Activity of DNA Topoisomerase II Janet E. Lindsley Analysis of Topoisomerase-DNA Interactions by Electrophoretic Mobility Shift Assay Stewart Shuman Filter Binding Assays for Topoisomerase-DNA Complexes Joaquim Roca DNA Topoisomerase I-Mediated Nicking of Circular Duplex DNA James J. Champoux Analysis of Cleavage Complexes Using Reactive Inhibitor Derivatives Kenneth N. Kreuzer, Catherine H. Freudenreich, and Yves Pommier Uncoupling of Topoisomerase-Mediated DNA Cleavage and Religation Anni H. Anderson, Kent Christiansen, and Ole Westergaard Synthesis and Use of DNA Containing a 5'-Bridging Phosphorothioate as a Suicide Substrate for Type I DNA Topoisomerases Alex B. Burgin, Jr Isolation of Covalent Enzyme-DNA Complexes Thomas C. Rowe, Dale Grabowski, and Ram Ganapathi ICE Bioassay: Isolating In Vivo Complexes of Enzyme to DNA Deepa Subramanian, Christine Sommer Furbee, and Mark T. Muller DNA Unwinding Test Using Eukaryotic DNA Topoisomerase I Linus L. Shen Drug-DNA Interactions David E. Graves Quinolone Interactions with DNA and DNA Gyrase Linus L.Shen Bactericidal Assays for Fluoroquinolones Thomas D. Gootz Drug Toxicity in E. coli Cells Expressing Human DNA Topoisomerase I Scott T. Taylor and Rolf Menzel Drug-Induced Cytotoxicity in Tissue Culture Cynthia E. Herzog and Leonard A. Zwelling Analysis of Drug Uptake-Flow Cytometry Grace Zoorob and Thomas Burke Cell Cycle Analysis of Drug-Treated Cells Frank Traganos, Gloria Juan, and Zbigniew Darzynkiewicz Assaying Drug-Induced Apoptosis Zbigniew Darzynkiewicz, Gloria Juan, and Frank Traganos Single-Strand Conformational Polymorphism Analysis of DNA Topoisomerases I and II Mary K. Danks PCR-Based Cloning of DNA Topoisomerase Genes Wai Mun Huang Topoisomerase II-Catalyzed Relaxation and Catenation of Plasmid DNA John M. Fortune and Neil Osheroff Topoisomerase II-Mediated Cleavage of Plasmid DNA D. Andrew Burden, Stacie J. Froelich-Ammon, and Neil Osheroff Drug-induced Stabilization of Covalent DNA Topoisomerase I-DNA Intermediates: DNA Cleavage Assays Paola Fiorani, Christine L. Hann, Piero Benedetti, and Mary-Ann Bjornsti Studying DNA Topoisomerase I-Targeted Drugs in the Yeast Saccharomyces cerevisiae Michael H. Woo, John R. Vance, and Mary-Ann Bjornsti Yeast Systems for Demonstrating the Transfer of Antitopoisomerase II Agents John L. Nitiss and Karen C. Nitiss Index