25 strains of staphylococci were isolated from 100 samples of milks that were clinic healthy cows in Shihezi area,They were identified to be staphylococcus aureus,staphylococcus epidermidis and staphylococcus pythogenesis.The result showed that staphylococcus aureus resistant to Enrofloxacin,Amoxicillin and lethality of small mouse was higher than that of staphylococcus epidermidis and staphylococcus pythogenesis.