核仁
H3K4me3
染色质免疫沉淀
组蛋白
细胞生物学
生物
染色质
组蛋白甲基化
基因表达
基因表达调控
遗传学
基因
DNA甲基化
发起人
细胞质
作者
Xiaolei Zhang,Guixin Zhu,Xuantao Su,Haitao Li,Wei Wu
标识
DOI:10.1016/j.bbrc.2018.09.098
摘要
Spindlin1 (SPIN1), a histone modification reader protein, was enriched in the cell nucleolus and facilitated rRNA expression. However, how SPIN1 localizes to the nucleolus and its functional role in rRNA gene expression remain unresolved. Here, we identified a nucleolar localization signal in the N-terminal region of SPIN1 that is essential for its enrichment and function in the nucleolus. We also discovered that, in addition to its H3K4me3 recognizing activity, the H3R8me2a-recognizing capacity of SPIN1 is also indispensable for stimulating rRNA expression. Chromatin immunoprecipitation results indicated that SPIN1 is required for the association or assembly of selective factor 1 (SL1) complex, probably facilitating the initiation of rDNA transcription through its H3 K4me3-R8me2a reader function.
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