Interactions of humic acid with pristine poly (lactic acid) microplastics in aqueous solution

) and free energy (E) suggest that adsorption of humic acid onto PLA is controlled by physisorption. We studied the effects of pH, ionic strength, and PLA concentration on the adsorption of humic acid onto PLA and demonstrated that electrostatic interactions and aggregation are important. The humic acid was characterized by Fourier-transform infrared (FTIR) spectroscopy, excitation-emission matrix (EEM) fluorescence spectroscopy, and parallel factor analysis (PARAFAC), before and after interacting with PLA. This set of analyses demonstrated that PLA caused alterations in the molecular structure of humic acid, primarily attributed to modifications in hydrogen bonding and hydrophobic interactions. Therefore, we hypothesize that the carboxylic groups of humic acid formed dimers in contact with PLA. This study provides new insights into the interactions between organic matter and a biodegradable microplastic in aqueous systems.