核糖核酸酶P
内啡肽酶
核糖核酸酶
核糖核酸酶MRP
核糖核酸
核糖核酸酶Ⅲ
核糖核酸酶H
小分子
细胞生物学
生物化学
生物
分子生物学
化学
RNA干扰
基因
作者
Noah A. Springer,Samantha M. Meyer,Amirhossein Taghavi,Raphael I. Benhamou,Yuquan Tong,Jessica L. Childs‐Disney,Matthew D. Disney
出处
期刊:Methods in Enzymology
日期:2023-01-01
卷期号:: 249-298
标识
DOI:10.1016/bs.mie.2023.06.006
摘要
Recently, a class of heterobifunctional small molecules called ribonuclease targeting chimeras (RiboTACs) have been developed that selectively induce degradation of RNAs in cells. These molecules function by recruiting latent ribonuclease (RNase L), an endoribonuclease involved in the innate immune response, to targeted RNA structures. The RiboTACs must activate RNase L in proximity to the RNA, resulting in cleavage of the RNA and downstream degradation. To develop and validate a new RiboTAC, several steps must be taken. First, small molecule activators that bind to RNase L must be identified. Next, since RNase L is only catalytically active upon ligand-induced homodimerization, the capability of identified small molecules to activate RNase L must be assessed. RNase L-activating small molecules should then be coupled to validated RNA-binding small molecules to construct the active RiboTAC. This RiboTAC can finally be assessed in cells for RNase L-dependent degradation of target RNAs. This chapter will provide several methods that are helpful to develop and assess RiboTACs throughout this process, including recombinant RNase L expression, methods to assess RNase L engagement in vitro such as saturation transfer difference nuclear magnetic resonance (STD NMR), an in vitro assay to assess activation of RNase L, and cellular methods to demonstrate RNase L-dependent cleavage.
科研通智能强力驱动
Strongly Powered by AbleSci AI