转移酶
反作用坐标
基质(水族馆)
糖基转移酶
化学
细胞内
酶
立体化学
生物化学
生物
计算化学
生态学
作者
Michael B. Lazarus,Jiaoyang Jiang,T.M. Gloster,Wesley F. Zandberg,Garrett E. Whitworth,David J. Vocadlo,Suzanne Walker
标识
DOI:10.1038/nchembio.1109
摘要
Visualization of the reaction coordinate undertaken by glycosyltransferases has remained elusive but is critical for understanding this important class of enzyme. Using substrates and substrate mimics, we describe structural snapshots of all species along the kinetic pathway for human O-linked β-N-acetylglucosamine transferase (O-GlcNAc transferase), an intracellular enzyme that catalyzes installation of a dynamic post-translational modification. The structures reveal key features of the mechanism and show that substrate participation is important during catalysis.
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