Study on the Adsorption Mechanism of Chitosan Magnetic Microspheres on BSA

Magnetic chitosan was prepared by cross-linkage of 1-chloro-2,3-epoxypropane with chitosan with 85% of degree of deacetylation,which was coated on ferroferric oxide magneton surface obtained by coprecipitation of ferrous ion and ferric ion in alkaline solution.The appearance,composition and thermal property of magnetic particles were analyzed by TEM,IR,DSC-TG and XRD,and the adsorption on BSA from BSA-disodium hydrogen phosphate-citric acid buffer was studied.Result showed that the adsorption of BSA was increased with the increase of pH,initial concentration of protein and incubation time,whereas temperature was a complicated factor.