微泡
细胞内
外体
生物发生
细胞生物学
体内
化学
蛋白质-蛋白质相互作用
蛋白质聚集
蛋白质生物合成
生物物理学
生物
生物化学
基因
小RNA
生物技术
作者
Nambin Yim,Seung Wook Ryu,Kyunghee Choi,Kwang Ryeol Lee,Seung‐Hee Lee,Hojun Choi,Jeongjin Kim,Mohammed R. Shaker,Woong Sun,Ji Ho Park,Daesoo Kim,Won Do Heo,Chulhee Choi
摘要
Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named 'exosomes for protein loading via optically reversible protein-protein interactions' (EXPLORs). By integrating a reversible protein-protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes. Treatment with protein-loaded EXPLORs is shown to significantly increase intracellular levels of cargo proteins and their function in recipient cells in vitro and in vivo. These results clearly indicate the potential of EXPLORs as a mechanism for the efficient intracellular transfer of protein-based therapeutics into recipient cells and tissues.
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