戊二醛
生物传感器
脂肪酶
电极
循环伏安法
材料科学
聚合物
膜
扫描电子显微镜
介电谱
化学
色谱法
化学工程
电化学
纳米技术
有机化学
复合材料
生物化学
物理化学
工程类
酶
作者
Jinal B. Thakkar,Dimpal J. Aghera,Bhavana Trivedi,C. Ratna Prabha
标识
DOI:10.1016/j.ijbiomac.2022.12.281
摘要
High levels of triglycerides in blood can harden and block the arteries increasing the risk of heart disease and strokes. Triglycerides are important constituents of oils and fats used in various foods. The triglyceride content in commercial preparations of oils is estimated using conventional methods. In the present study, an electrochemical biosensor with lipase immobilized novel conductive polymer film has been developed for estimating triglyceride content in a variety of products. The portable biosensor can bring down the detection costs dramatically and can be used for varied purposes. It is based on cyclic voltammetry and has a three-electrode configuration system. Glassy carbon electrode is functionalized with nanoparticles embedded in polyethyleneimine and lipase is immobilized using glutaraldehyde. The strategy increases the electrochemical conductance manifold and overcomes the hindrance to lipase posed by membranes as it is oriented on the outside of the membrane. Thus, it increases the sensitivity and selectivity of detection. Results of scanning electron microscopy and FT-IR spectroscopy were used for characterizing the electrode surface. Linear range of the electrode for triglycerides is 100–500 mg/dL. The sensor was used successfully to determine triglyceride content in several real samples and the average recovery values lie from 95.47 % to 101.05 %.
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