鲍曼不动杆菌
抗菌剂
微生物学
类胡萝卜素
生物膜
屎肠球菌
铜绿假单胞菌
金黄色葡萄球菌
抗菌肽
粪肠球菌
肺炎克雷伯菌
细菌
肽
抗生素
抗生素耐药性
化学
生物
大肠杆菌
生物化学
基因
遗传学
作者
Biswajit Mishra,Guangshun Wang
出处
期刊:Biofouling
[Informa]
日期:2017-07-04
卷期号:33 (7): 544-555
被引量:42
标识
DOI:10.1080/08927014.2017.1332186
摘要
Infections on implanted medical devices are a challenging problem, especially when bacteria form difficult-to-treat biofilms. Antimicrobial peptides are considered to be a solution due to their potency against antibiotic-resistant superbugs. Previously, the authors’ laboratory demonstrated the prevention of staphylococcal biofilm formation in an animal catheter model by injecting merecidin (formerly known as 17BIPHE2), a peptide engineered based on the only human cathelicidin. This study documents an alternative solution via covalent immobilization of FK-16, amino acid sequence FKRIVQRIKDFLRNLV-amide, which corresponds to the major antimicrobial region (residues 17–32) of LL-37. FK-16 is superior to the longer peptide LL-37 in terms of synthesis cost and the shorter peptide KR-12 in terms of activity spectrum. Indeed, the FK16-coated titanium surface showed a broad-spectrum activity against the ESKAPE pathogens, including Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species. It also demonstrated anti-adhesion and biofilm inhibition capabilities against both S. aureus and E. coli.
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