Screening of Yeast Strains Producing Ergosterol

A high Ergosterol-producing strain, Saccharomyces cerevisiae YN2 was selected from 12 yeasts in our laboratory. The biomass of cells reached 5.63mg/ml in fermentation liquid and the content of ergosterol reached 1.82% in dry weight of cells,when the yeast had been cultured at 281 for 60 hours in shake flask containing YEPD media. The UV-absorption course of ergosterol showed that the UV ABS of ergosterol produced by YN2 had the very same curve to standard ergosterol agent.