CD90型
滑膜
胶原酶
CD44细胞
川地68
病理
滑膜切除术
成纤维细胞
巨噬细胞
表型
细胞培养
生物
类风湿性关节炎
关节炎
细胞
细胞生物学
医学
免疫学
免疫组织化学
体外
基因
遗传学
酶
生物化学
作者
Sanna Rosengren,David L. Boyle,Gary S. Firestein
出处
期刊:Methods in molecular medicine
日期:2007-01-01
卷期号:: 365-375
被引量:89
标识
DOI:10.1007/978-1-59745-401-8_24
摘要
The study of fibroblast-like synoviocytes (FLS) has yielded important insights into the pathogenic mechanisms of rheumatoid arthritis. FLS can be cultured from synovial tissue obtained at joint replacement surgery, synovectomy, or synovial biopsy. After collagenase digestion, adherent cells consist mainly of synovial fibroblasts and synovial macrophages. Proliferating FLS are enriched by repeated passage and comprise >95% of cells by passage 3. Because of cell senescence, use of FLS lines after passage 9 is generally not recommended. FLS in culture have a distinct phenotype with regard to morphology, ultrastructure, surface phenotype, and function. Surface markers that can be used to characterize FLS include positive staining for VCAM-1, CD44, CD55, CD90 (Thy-1), and cadherin-11, coupled with the absence of macrophage markers such as CD14 or CD68.
科研通智能强力驱动
Strongly Powered by AbleSci AI