Response surface methodology for optimization of enzymatic extraction of polysaccharide from Laver

Using cellulase assisted extraction of Laver polysaccharide,with the enzyme concentration,temperature,hydrolysis time and pH as a response surface design variables. The result showed that the optimum hydrolysis conditions for a considerably enhanced extraction yield of polysaccharide were determined as follows: 1. 5% of enzyme concentration,51 ℃,pH 5. 0 and 80 min of extraction time. Under this condition,the polysaccharide yield is 19. 46%.