效应器
VI型分泌系统
分泌物
内质网
周质间隙
铜绿假单胞菌
细胞生物学
毒力
未折叠蛋白反应
自噬
生物
磷脂酶
微生物学
三型分泌系统
细菌
生物化学
大肠杆菌
基因
酶
细胞凋亡
遗传学
作者
Feng Jiang,X. Wang,Bei Wang,Lihong Chen,Zhendong Zhao,Nicholas R. Waterfield,Guowei Yang,Qi Jin
出处
期刊:Cell Reports
[Elsevier]
日期:2016-08-01
卷期号:16 (6): 1502-1509
被引量:86
标识
DOI:10.1016/j.celrep.2016.07.012
摘要
Pseudomonas aeruginosa is an opportunistic pathogen that regularly causes nosocomial infections in hospitalized patients. The type VI secretion system (T6SS) is responsible for the secretion of numerous virulence effector proteins that can both interfere with competing microbes and manipulate host cells. Here, we report a detailed investigation of a P. aeruginosa H2-T6SS-dependent phospholipase effector, TplE, which acts as a trans-kingdom toxin. Delivery of TplE to the periplasmic space of rival bacteria leads to growth inhibition. Importantly, TplE, also contains a eukaryotic PGAP1-like domain, which targets the host ER apparatus, ultimately leading to disruption of the ER. TplE activity leads to the activation of the unfolded protein response (UPR) through the IRE1α-XBP1 pathway, enhancing autophagic flux. These findings indicate that this T6SS-delivered phospholipase effector is active against both prokaryotic and eukaryotic cellular targets, highlighting the T6SS as a versatile weapon in the Pseudomonas arsenal.
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