金黄色葡萄球菌
异牡荆素
凝固酶
纤维蛋白原
微生物学
纤维蛋白
人类病原体
生物
化学
生物化学
葡萄球菌
免疫学
细菌
遗传学
基因
抗氧化剂
类黄酮
牡荆素
作者
Hua Xiang,Panpan Yang,Li Wang,Jiaxin Li,Tiedong Wang,Jun-Ze Xue,Dacheng Wang,Huiqin Ma
出处
期刊:Journal of Microbiology and Biotechnology
[Journal of Microbiology and Biotechnology]
日期:2021-10-28
卷期号:31 (10): 1350-1357
被引量:6
标识
DOI:10.4014/jmb.2105.05013
摘要
Staphylococcus aureus (S. aureus) is a major pathogen that causes human pneumonia, leading to significant morbidity and mortality. S. aureus coagulase (Coa) triggers the polymerization of fibrin by activating host prothrombin, which then converts fibrinogen to fibrin and contributes to S. aureus pathogenesis and persistent infection. In our research, we demonstrate that isovitexin, an active traditional Chinese medicine component, can inhibit the coagulase activity of Coa but does not interfere with the growth of S. aureus. Furthermore, we show through thermal shift and fluorescence quenching assays that isovitexin directly binds to Coa. Dynamic simulation and structure-activity relationship analyses suggest that V191 and P268 are key amino acid residues responsible for the binding of isovitexin to Coa. Taken together, these data indicate that isovitexin is a direct Coa inhibitor and a promising candidate for drug development against S. aureus infection.
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