Nematode families establishment and pathogenicity evaluation of Bursaphelenchus xylophilus and B.mucronatus Strains

The stable apparent characters and same genetic background is the important basis to study the nematode pathogenic molecular mechanism.In order to obtain the nematode families with the same genetic background and stable virulence of Bursaphelenchus xylophilus and B.mucronatus,the nematode families were established by the method of larvae-larvae pair co-culture.A number of the nematode families were obtained with the success rate 13.3 to 20 percent and it spent 10 to 44 days.The virulence analysis showed that there was a significant difference amnong the nematode families.According to the rate of infection,susceptibility index,duration of inoculated pine seedlings,the B.xylophilus family AN19#F1 was the most virulent and all B.mucronatus families from AN5# strain were avirulent.