化学
DPPH
抗氧化剂
食品科学
阿布茨
乳酸
糖苷键
多糖
半乳糖
干酪乳杆菌
细菌
生物化学
生物
发酵
遗传学
酶
作者
Xiao-Yu Yang,Yi-Ming Ren,Lina Zhang,Zhiwen Wang,Liang Li
标识
DOI:10.1016/j.lwt.2021.111811
摘要
This is the first report on the structure and antioxidant property of exopolysaccharide (EPS), which was extracted from soybean protein isolate gels induced by lactic acid bacteria (LAB). EPSs were named AEPS ( L. acidophilus ), CEPS ( L. casei ), MEPS ( L. mesenteroides ) and LEPS ( L. lactis ). EPS was primarily composed of glucose, galactose and glucuronic acid, existed as random coils, and appeared as round lumps and chains. NMR showed that EPS was composed of α-glycosidic bond (δ5.0–6.0 ppm) and β-glycosidic bonds (δ4.5–5.0 ppm). The peaks near at 1644 cm −1 and 590 cm −1 correspond to uronic acid and sulphate groups, respectively. CEPS had the highest ability to scavenge DPPH (58.98 ± 1.54%), hydroxyl (64.89 ± 1.46%) and ABTS (64.89 ± 1.46%) free radicals. The monosaccharide composition-antioxidant activity relationships were identified using multiple linear regression analysis. This study could help promote the application of both EPS and LAB in functional soybean products. • The first time to study EPS produced by LAB involved in soybean protein gel. • NMR showed that EPS were a pyranose composed of α-and β-glycosidic bonds. • The EPS production from L. casei showed the highest scavenging activities. • Good linear correlations among scavenging activities and structure were found. • Multiple linear regression analysis was used to analyse relationships.
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