展青霉素
赭曲霉毒素A
桔霉素
黄曲霉毒素
真菌毒素
检出限
色谱法
化学
食品科学
玉米赤霉烯酮
巴氏杀菌
链霉亲和素
生物素
生物化学
作者
Xiaoqian Tang,Qi Zhang,Maria Isabel Pividori,Zhaowei Zhang,Jean Louis Marty,Gaëlle Catanante
出处
期刊:Biosensors
[MDPI AG]
日期:2022-01-23
卷期号:12 (2): 59-59
被引量:8
摘要
Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2.7~139.8 ng/mL in the buffer and 7.07~359.5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B1 (AFB1), citrinin or tenuazonic acid (TEA). The limit of detection was 0.19 ng/mL in the buffer and 0.36 ng/mL in the real sample. The recoveries were 83.3% to 107.1%, with a satisfactory RSD value from 6.5% to 7.5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT determination in apple juice.
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