免疫标记
线粒体膜间隙
线粒体
细胞生物学
生物
膜间隙
核糖核酸
基因
MT-RNR1型
生物化学
细菌外膜
免疫学
免疫组织化学
大肠杆菌
作者
Anna Smirnova,Ludovic Richert,Alexandre Smirnov,Yves Mély,Ivan Tarassov
出处
期刊:Springer eBooks
[Springer Nature]
日期:2021-01-01
卷期号:: 157-173
被引量:1
标识
DOI:10.1007/978-1-0716-1270-5_11
摘要
Mitochondria have complex ultrastructure which includes continuous subcompartments, such as matrix, intermembrane space, and two membranes, as well as focal structures, such as nucleoids, RNA granules, and mitoribosomes. Comprehensive studies of the spatial distribution of proteins and RNAs inside the mitochondria are necessary to understand organellar gene expression processes and macromolecule targeting pathways. Here we give examples of distribution analysis of mitochondrial proteins and transcripts by conventional microscopy and the super-resolution technique 3D STORM. We provide detailed protocols and discuss limitations of immunolabeling of mitochondrial proteins and newly synthesized mitochondrial RNAs by bromouridine incorporation and single-molecule RNA FISH in hepatocarcinoma cells.
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