血小板源性生长因子受体
血小板衍生生长因子
生长因子
分泌物
成纤维细胞
细胞生物学
细胞生长
细胞培养
生物
刺激
内分泌学
分子生物学
化学
内科学
受体
生物化学
医学
遗传学
作者
Elaine W. Raines,Steven K. Dower,Russell Ross
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1989-01-20
卷期号:243 (4889): 393-396
被引量:626
标识
DOI:10.1126/science.2783498
摘要
Both interleukin-1 (IL-1) and platelet-derived growth factor (PDGF) induce proliferation of cultured fibroblasts and smooth muscle cells. These polypeptide mediators are released by activated macrophages and other cell types in response to injury and are thought to have a role in tissue remodeling and a number of pathologic processes. Analysis of the kinetics of [3H]thymidine incorporation by cultured fibroblasts demonstrated that the response to IL-1 is delayed approximately 8 hours relative to their response to PDGF. IL-1 transiently stimulated expression of the PDGF A-chain gene, with maximum induction after approximately 2 hours. Subsequent synthesis and release of PDGF activity into the medium was detected as early as 4 hours after IL-1 stimulation, and downregulation of the binding site for the PDGF-AA isoform of PDGF followed PDGF-AA secretion. Antibodies to PDGF completely block the mitogenic response to IL-1. Therefore, the mitogenic activity of IL-1 for fibroblasts and smooth muscle cells appears to be indirect and mediated by induction of the PDGF A-chain gene.
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