microRNA‐8514‐5p regulates adipokinetic hormone/corazonin‐related peptide receptor to affect development and reproduction of Plutella xylostella

菜蛾 生物 小菜蛾 拮抗剂 脂肪运动激素 蜕皮激素受体 细胞生物学 小RNA 受体 基因 生物化学 神经肽 核受体 转录因子 幼虫 植物
作者
Senbo Pang,Shuo Wang,Muhammad Asad,Jiajing Yu,Guifang Lin,Jinzhi Chen,Cuiying Sun,Pengrong Huang,Yanpeng Chang,Hui Wei,Guang Yang
出处
期刊:Pest Management Science [Wiley]
卷期号:80 (10): 5377-5387 被引量:3
标识
DOI:10.1002/ps.8267
摘要

Abstract BACKGROUND Dicer1 plays a crucial role in regulating the development and reproduction of insects. Knockout of Dicer1 causes pupal deformity, low eclosion and low fecundity in Plutella xylostella , but the mechanism behind this phenomenon is not clear. This study aims to identify differentially‐expressed genes and miRNAs in the Dicer1 ‐knockout strain (Δ PxDcr‐1 ) and assess their impact on the reproduction and development of P. xylostella . RESULTS The knockout of Dicer1 affected the expression of genes including the adipokinetic hormone/corazonin‐related peptide receptor ( PxACPR ). The expression of PxACPR was upregulated, and the expression of miR‐8514‐5p was downregulated in Δ PxDcr‐1 of P. xylostella . The dual luciferase reporter assay and pull‐down assay showed that miR‐8514‐5p bound to PxACPR in vitro and in vivo . The expression profiles demonstrated a negative correlation between PxACPR mRNA and miR‐8514‐5p in different developmental stages of the wild‐type strain. Both the miR‐8514‐5p agomir and double‐stranded RNA of ACPR (ds PxACPR ) injected into the pre‐pupae inhibited the mRNA level of PxACPR , causing high mortality and deformity of pupae, and low fecundity and hatching rate, which were consistent with the phenotype of Δ PxDcr‐1 . The injection of miR‐8514‐5p antagomir caused a similar phenotype to the injection of miR‐8514‐5p agomir. Additionally, the injection of miR‐8514‐5p antagomir significantly rescued the phenotype caused by ds PxACPR . CONCLUSION These results indicate that miR‐8514‐5p affects the development and reproduction of P. xylostella by regulating PxACPR , and the homeostasis of PxACPR expression is essential for the development and reproduction of P. xylostella. © 2024 Society of Chemical Industry.
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