microRNA‐8514‐5p regulates adipokinetic hormone/corazonin‐related peptide receptor to affect development and reproduction of Plutella xylostella

菜蛾 生物 小菜蛾 拮抗剂 脂肪运动激素 蜕皮激素受体 细胞生物学 小RNA 受体 基因 生物化学 神经肽 核受体 转录因子 幼虫 植物
作者
Senbo Pang,Shuo Wang,Muhammad Asad,Jiajing Yu,Guifang Lin,Jinzhi Chen,Cuiying Sun,Pengrong Huang,Yanpeng Chang,Hui Wei,Guang Yang
出处
期刊:Pest Management Science [Wiley]
卷期号:80 (10): 5377-5387 被引量:3
标识
DOI:10.1002/ps.8267
摘要

Abstract BACKGROUND Dicer1 plays a crucial role in regulating the development and reproduction of insects. Knockout of Dicer1 causes pupal deformity, low eclosion and low fecundity in Plutella xylostella , but the mechanism behind this phenomenon is not clear. This study aims to identify differentially‐expressed genes and miRNAs in the Dicer1 ‐knockout strain (Δ PxDcr‐1 ) and assess their impact on the reproduction and development of P. xylostella . RESULTS The knockout of Dicer1 affected the expression of genes including the adipokinetic hormone/corazonin‐related peptide receptor ( PxACPR ). The expression of PxACPR was upregulated, and the expression of miR‐8514‐5p was downregulated in Δ PxDcr‐1 of P. xylostella . The dual luciferase reporter assay and pull‐down assay showed that miR‐8514‐5p bound to PxACPR in vitro and in vivo . The expression profiles demonstrated a negative correlation between PxACPR mRNA and miR‐8514‐5p in different developmental stages of the wild‐type strain. Both the miR‐8514‐5p agomir and double‐stranded RNA of ACPR (ds PxACPR ) injected into the pre‐pupae inhibited the mRNA level of PxACPR , causing high mortality and deformity of pupae, and low fecundity and hatching rate, which were consistent with the phenotype of Δ PxDcr‐1 . The injection of miR‐8514‐5p antagomir caused a similar phenotype to the injection of miR‐8514‐5p agomir. Additionally, the injection of miR‐8514‐5p antagomir significantly rescued the phenotype caused by ds PxACPR . CONCLUSION These results indicate that miR‐8514‐5p affects the development and reproduction of P. xylostella by regulating PxACPR , and the homeostasis of PxACPR expression is essential for the development and reproduction of P. xylostella. © 2024 Society of Chemical Industry.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
1秒前
一与余发布了新的文献求助10
1秒前
ddaa完成签到,获得积分10
1秒前
赘婿应助科研通管家采纳,获得10
3秒前
kk应助科研通管家采纳,获得10
3秒前
3秒前
3秒前
斯文败类应助科研通管家采纳,获得10
3秒前
3秒前
3秒前
3秒前
wuwei91完成签到,获得积分10
3秒前
大壮发布了新的文献求助10
3秒前
6秒前
wuwei91发布了新的文献求助10
8秒前
9秒前
邓涛完成签到,获得积分10
10秒前
10秒前
pluto应助一与余采纳,获得10
11秒前
12秒前
王自信发布了新的文献求助10
12秒前
今后应助666采纳,获得10
12秒前
13秒前
吕佳完成签到 ,获得积分10
13秒前
14秒前
吕邓宏发布了新的文献求助10
15秒前
16秒前
英姑应助Spring采纳,获得10
16秒前
17秒前
Flipped完成签到,获得积分20
17秒前
策略完成签到,获得积分10
17秒前
明时发布了新的文献求助10
17秒前
喵喵喵发布了新的文献求助10
18秒前
18秒前
Hello应助王自信采纳,获得10
19秒前
曹帅发布了新的文献求助10
19秒前
罗小黑发布了新的文献求助10
19秒前
mnwkwcj发布了新的文献求助10
20秒前
41应助悦耳的海燕采纳,获得10
21秒前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Social Research Methods (4th Edition) by Maggie Walter (2019) 2390
A new approach to the extrapolation of accelerated life test data 1000
北师大毕业论文 基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 390
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Robot-supported joining of reinforcement textiles with one-sided sewing heads 360
Atlas of Interventional Pain Management 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4005837
求助须知:如何正确求助?哪些是违规求助? 3545744
关于积分的说明 11293858
捐赠科研通 3281777
什么是DOI,文献DOI怎么找? 1809781
邀请新用户注册赠送积分活动 885544
科研通“疑难数据库(出版商)”最低求助积分说明 811017