Process Optimization for Enzymatic Hydrolysis Condition to Coix Seed Protein by Protease

In order to optimize the optimal condition of hydrolysis for coix seed protein by protease,taking nitrogen solubility index and degree of hydrolysis as the indicator,select the best hydrolysis protease from the neutrase,alcalase,papain,and trypsin.Finally,identify alcalase is the best hydrolysis protease.Based on the single factor experiment and second-order regression rotation design,established the protease hydrolysis mathematical model.The results show that the optimal condition of alcalase is:substrate concentration is 39.95 g/L,the protease dosage is 1 201.75 U/g,temperature is 54.61℃,pH is 7.99,and time is 4 h.Under the optimal conditions,nitrogen solubility index of coix seed protein hydrolyzed by protease can reach 95.79%.