生物
基因组
进化生物学
放线菌门
基因
计算生物学
遗传学
16S核糖体RNA
作者
Yaxi Wang,Larry A. Gallagher,Pia A. Andrade,Andi Liu,Ian R. Humphreys,Serdar Turkarslan,Kevin J. Cutler,Mario L. Arrieta-Ortiz,Yaqiao Li,Matthew C. Radey,Jeffrey S. McLean,Qian Cong,David Baker,Nitin S. Baliga,S. Brook Peterson,Joseph D. Mougous
出处
期刊:Cell
[Elsevier]
日期:2023-10-01
卷期号:186 (22): 4803-4817.e13
被引量:1
标识
DOI:10.1016/j.cell.2023.08.017
摘要
Patescibacteria, also known as the candidate phyla radiation (CPR), are a diverse group of bacteria that constitute a disproportionately large fraction of microbial dark matter. Its few cultivated members, belonging mostly to Saccharibacteria, grow as epibionts on host Actinobacteria. Due to a lack of suitable tools, the genetic basis of this lifestyle and other unique features of Patescibacteira remain unexplored. Here, we show that Saccharibacteria exhibit natural competence, and we exploit this property for their genetic manipulation. Imaging of fluorescent protein-labeled Saccharibacteria provides high spatiotemporal resolution of phenomena accompanying epibiotic growth, and a transposon-insertion sequencing (Tn-seq) genome-wide screen reveals the contribution of enigmatic Saccharibacterial genes to growth on their hosts. Finally, we leverage metagenomic data to provide cutting-edge protein structure-based bioinformatic resources that support the strain Southlakia epibionticum and its corresponding host, Actinomyces israelii, as a model system for unlocking the molecular underpinnings of the epibiotic lifestyle.
科研通智能强力驱动
Strongly Powered by AbleSci AI