Modulation of heat shock protein 70 (HSP70) gene expression ex vivo in response to heat stress in chicken

The present study was conducted to assess the effect of exposure to heat stress on the HSP70 gene expression pattern ex vivo in chickens. The adult healthy birds (n = 15) grouped into three replicates of n = 5 each were used to isolate peripheral blood mononuclear cells (PBMCs). The PBMCs were subjected to heat stress at 42 °C for 1 h, and cells without heat stress (NHS) were taken as control. The cells were seeded in 24 well plates and incubated in a humidified incubator at 37 °C under 5% CO2 for recovery. HSP70 expression kinetics were evaluated at 0, 2, 4, 6, and 8 h of the recovery period. Compared with NHS, the expression pattern of HSP70 was upregulated gradually from 0 to 4 h with peak (p < 0.05) expression recorded at 4 h of recovery time. mRNA expression of HSP70 escalated in a time-dependent manner from 0 to 4 h of heat exposure and thereafter exhibited a gradually decreasing pattern till 8 h of the recovery period. The findings from this study highlight the protective role of HSP70 against the deleterious effects of heat stress in chicken PBMCs. Further, the study demonstrates the possible use of PBMCs as a cellular system in assessing the heat stress effect in chickens ex vivo.