We used waste cotton?cottonseed shell?rice straw and wheat straw as culture media and cultured the volvariella volvacea.The result showed that the hyphae of volvariella volvacea grew the fastest and the output was the highest in the waste cotton(the biological efficiency was 32.02%).But in the wheat straw the hyphae of Volvariella Volvacea grew the slowest and the output was the lowest(The biological efficiency was 8.82%).So we could think that the waste cotton was the best medium for the growth of volvariella volvacea.