Microscopic observation of vacuoles isolated and purified from tobacco leaves

Observing vacuoles isolated and purified from tabacco leaves under optic and electronic microscopes, we obtained photographs of protoplasts, process of vacuoles released from protoplasts and vacuoles after purification. The results show that the method we used could obtain complete protoplasts and vacuoles, and the central vacuole is the largest organelle in one mature plant cell.