间充质干细胞
脐带
干细胞
刺激
免疫学
受体
淋巴细胞
生物
细胞生物学
内分泌学
生物化学
作者
Xiao-Dun Liu,Dong Liu,Chuanbao Zang,Haiyan Zhang,Ming Yi,Ling-Long Chi,Bai-Jun Shen,Dong Li
出处
期刊:PubMed
日期:2014-06-01
卷期号:22 (3): 605-11
被引量:1
标识
DOI:10.7534/j.issn.1009-2137.2014.03.006
摘要
This study was objective to explore the effect of IFN-γ on immunosuppressive capability of mesenchymal stem cells (MSC) derived from umbilical cord. The immunomodulating capability of MSC was changed by stimulating cell surface receptors like Toll-like receptors (TLR). The inhibition of T-lymphocyte proliferation by MSC was tested via cell co-cultures. Further RT-PCR and ELISA were performed to examine the expression changes in gene and protein level. The results showed that the IFN-γ could promote the immunosuppressive effect of umbilical cord derived MSC. IFN-γ-stimulated MSC could suppress the proliferation of T cells more effectively. IFN-γ stimulation up-regulated the expression of immunosuppressive genes like IDO1, COX2, HLA-G, and soluble suppressive proteins such as HLA-G, KYN, IL10, PGE2 of MSC. And the immuno suppression capability of IFN-γ-stimulated MSC was 2-7 folds higher than control in MSC and lymphocyte co-culture tests. It is concluded that IFN-γ can effectively enhance the immunosuppressive capability of MSC.
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